Differential localization of dense granule antigens in tachyzoites and bradyzoites during in vitro culture. Localizations of NcGRA1, -2, and -7 in tachyzoite cultures were identical, as exemplified by NcGRA1 (A to C). During in vitro stage conversion, a strong shift of labeling toward the vacuole periphery was noted for NcGRA1 (D to F) and NcGRA7 (J to L), and also to a lesser extent for NcGRA2 (G to I).