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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (silver stain) and corresponding immunoblots of tachyzoite (T) and bradyzoite (B) extracts. Identical numbers of parasites were loaded. Note different banding patterns obtained in bradyzoite and tachyzoite extracts by silver staining and immunoblotting using an anti-N. caninum antiserum (Neo) (20), the down-regulation of NcSAG1 and NcSRS2 expression, and the appearance of NcBAG1 expression in bradyzoites.
