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. Author manuscript; available in PMC: 2013 Sep 6.
Published in final edited form as: Neuron. 2012 Sep 6;75(5):810–823. doi: 10.1016/j.neuron.2012.07.007

Figure 4. Clathrin is required for somatodendritic sorting of TfR.

Figure 4

(A) Neurons (DIV10) co-transfected with plasmids encoding GFP-tagged TfR-WT (grayscale negative, top panels) together with empty vector (control) or vector encoding T7-epitope-tagged clathrin heavy chain hub were immunostained for the T7 epitope, MAP2 and Ank-G. GFP fluorescence is shown in these panels and the corresponding immunostaining for T7, MAP2 and Ank-G is shown in Figure S4B. Arrow points to AIS and arrowheads indicate the axon in each neuron. Scale bars: 20 µm.

(B) Images of axons (top panels) and dendrites (bottom panels) magnified 5X from orange and magenta boxes, respectively, in A. TfR-GFP (green), MAP2 (red), and merged images are shown. Yellow in the merged images indicates co-localization.