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. Author manuscript; available in PMC: 2013 Sep 6.
Published in final edited form as: Neuron. 2012 Sep 6;75(5):810–823. doi: 10.1016/j.neuron.2012.07.007

Figure 6. Interaction with µ1A is required for somatodendritic sorting of neuronal glutamate receptor proteins.

Figure 6

(A) Y2H analysis of the interaction of the cytosolic domains of mGluR1 (residues 841–1199), NR2A (1304–1464), NR2B (1315–1484), GluR1 (827–906), GluR2 (834–883) or TGN38 (324–353) (positive control) with WT or W408S mutant forms of µ1A, performed as described in the legend to Figure 2B.

(B) Neurons (DIV10) co-expressing combinations of GFP-tagged mGluR1, NR2A, NR2B or GluR1, super-ecliptic pHluorin (SEP)-tagged GluR2 or untagged NgCAM with HA-tagged µ1A-WT or µ1A-W408S were immunostained for the HA epitope and Ank-G. The GFP or SEP signal was enhanced by immunostaining with antibody to GFP. NgCAM was also detected by immunostaining. GFP, SEP and NgCAM immunostaining is shown in these panels and the corresponding immunostaining for HA and Ank-G is shown in Figure S4C. The distribution of all glutamate receptor proteins and NgCAM is shown in grayscale negative images. Arrow points to the AIS and arrowheads to the axon in each neuron. Scale bars: 20 µm.