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. Author manuscript; available in PMC: 2013 Sep 19.

Published in final edited form as: Anal Chim Acta. 2012 Jul 13;743C:125–130. doi: 10.1016/j.aca.2012.06.054

Selective reagent delivery can also be used in a step-wise fashion. Calcein-AM (green) and propidium iodide (Red) staining of Ramos cells selectively treated with staurosporine for 24 hours. Cells under the reagent stream (A) were dead (11% viable) while viability of the untreated cells is high (90% viable). The fluidic system can be reversed so that buffer is loaded in the center stream and reagent streams are on the outside of the reagent channel (B). In this case, the outer portions of the culture chamber are treated with staurosporine (1% viable) and the center of the channel, under the reagent stream, is healthy (92% viable). This chip can therefore be used to locally treat cells and then globally stain them for analysis.

Selective reagent delivery can also be used in a step-wise fashion. Calcein-AM (green) and propidium iodide (Red) staining of Ramos cells selectively treated with staurosporine for 24 hours. Cells under the reagent stream (A) were dead (11% viable) while viability of the untreated cells is high (90% viable). The fluidic system can be reversed so that buffer is loaded in the center stream and reagent streams are on the outside of the reagent channel (B). In this case, the outer portions of the culture chamber are treated with staurosporine (1% viable) and the center of the channel, under the reagent stream, is healthy (92% viable). This chip can therefore be used to locally treat cells and then globally stain them for analysis.