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. 2004 Jan;72(1):515–526. doi: 10.1128/IAI.72.1.515-526.2004

FIG. 7.

FIG. 7.

Expression of M. tuberculosis rpf homologues in a late-stationary-phase (4-month-old) Erdman culture. RNA was prepared from Erdman grown in 7H9 medium, and 5 μg of total RNA was reverse transcribed and serially diluted fivefold for use as template in PCRs to amplify each of the rpf-like genes. Primers, as described in Materials and Methods, were used to amplify portions of Rv0867c (lanes 1 to 4), Rv1009 (lanes 5 to 8), Rv1884c (lanes 9 to 12), Rv2389c (lanes 13 to 16), and Rv2450c (lanes 17 to 20). Lane 21 is a negative control containing no template, and lane 22 shows amplification of 23S rRNA from the same cDNA template used to amplify the rpf genes. Lanes 23 to 28 contain non-reverse-transcribed RNA as the template, using primers to amplify Rv2389c (lane 23), Rv1009 (lane 24), Rv2450c (lane 25), Rv1884c (lane 26), Rv0867c (lane 27), and 23S rRNA (lane 28). A 50-bp ladder (Invitrogen) is shown to the left of each series of samples.