FIG. 8.

In vivo expression of M. tuberculosis rpf-like genes. RNA was prepared from lungs of mice infected with a clinical strain of M. tuberculosis, and total RNA (equivalent of 1.25 μg per PCR mixture) was reverse transcribed and used as the template in PCRs to amplify each of the rpf-like genes or to amplify 23S rRNA, as indicated by the gene designation above each lane. Also shown are the results of PCR amplification using RNA which had not been reverse transcribed (No RT), employing 23S rRNA primers, for both lung 1 and lung 2. The far right lane of each gel shows the results of PCR amplification using 23S rRNA primers with water (H₂O) as template.