TABLE 2.
Plasmids and strains used in this study
| Plasmid or strain | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pCR2.1 | Ampr; cloning vector for PCR products | Invitrogen |
| pjsc284 | Hygr; cosmid vector for cloning allelic exchange substrates; has oriE, single λ-cos site, PacI site for introduction into shuttle phasmid; res-hyg-res cassette flanked by γδ-res sites | Derivative of pYUB854, 1 |
| pMV261 | Kanr; pMV206 shuttle plasmid with hsp60 promoter | 33 |
| pMV306 | Kanr; site-specific integrating shuttle vector | 33 |
| phAE87 | TM4-based phasmid shuttle vector with temperature-sensitive regulon | 2 |
| pjmt17 | Hygr; pjsc284 containing flanking sequences of Rv2389c gene | This study |
| pjmt18 | Hygr; pjsc284 containing flanking sequences of Rv1009 gene | This study |
| pjmt18A | Hygr; pjsc284, but containing revised flanking sequence of Rv1009 gene | This study |
| pjmt19 | Hygr; pjsc284 containing flanking sequences of Rv2450c gene | This study |
| pjmt20 | Hygr; pjsc284 containing flanking sequences of Rv1884c gene | This study |
| pjmt21 | Hygr; pjsc284 containing flanking sequences of Rv0867c gene | This study |
| Strains | ||
| mc2155 | M. smegmatis | 32 |
| Erdman | M. tuberculosis | Trudeau Institute (Saranac Lake, N.Y.) |
| mc23125 | Erdman ΔRv2389c::res-hyg-res | This study |
| mc23126 | Erdman ΔRv1009::res-hyg-res | This study |
| mc23126A | Erdman ΔRv1009::res-hyg-res; revised construct | This study |
| mc23127 | Erdman ΔRv2450c::res-hyg-res | This study |
| mc23128 | Erdman ΔRv1884c::res-hyg-res | This study |
| mc23129 | Erdman ΔRv0867c::res-hyg-res | This study and reference 1a |
| mc23301 | mc23126 (attB::Rv1009) | This study |
| mc23302 | mc23126 (attB::ksgA) | This study |
| mc23301A | mc23126A (attB::Rv1009) | This study |
| mc23302A | mc23126A (attB::ksgA) | This study |
a
In reference 1, this strain is designated mc23510.