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. Author manuscript; available in PMC: 2012 Sep 12.

Published in final edited form as: Gastroenterology. 2008 Nov 1;136(3):1060–1069. doi: 10.1053/j.gastro.2008.10.025

Canalicular excretion of CGamF, but not CMFDA is reduced in Atp8b1 knockdown rat hepatocytes. The cells were treated with PBS, scrambled Ad-siRNA (Ctrl Ad-siRNA), or ATP8B1 Ad-siRNA2 and cultured for 5 days in collagen sandwich gel configuration. A, Cells were incubated with CGamF or CMFDA, and visualized by confocal microscopy. B, A quantitative measurement of the mean fluorescent CGamF intensity in canaliculi was obtained using Image J software by delineating the fluorescent canaliculi from the surrounding cells. Data represent the means ±SD of 3 independent experiments, *p<0.05. Bar, 10µm.

Canalicular excretion of CGamF, but not CMFDA is reduced in Atp8b1 knockdown rat hepatocytes. The cells were treated with PBS, scrambled Ad-siRNA (Ctrl Ad-siRNA), or ATP8B1 Ad-siRNA2 and cultured for 5 days in collagen sandwich gel configuration. A, Cells were incubated with CGamF or CMFDA, and visualized by confocal microscopy. B, A quantitative measurement of the mean fluorescent CGamF intensity in canaliculi was obtained using Image J software by delineating the fluorescent canaliculi from the surrounding cells. Data represent the means ±SD of 3 independent experiments, *p<0.05. Bar, 10µm.