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. 2004 Jan;72(1):277–283. doi: 10.1128/IAI.72.1.277-283.2004

FIG. 3.

FIG. 3.

Western blot analysis of patient sera. (A) Western blot of a total-membrane preparation of iron-stressed N. gonorrhoeae probed with patient sera diluted 1:150. (B) Western blot of a total membrane preparation of iron-stressed N. meningitidis probed with the same dilution of patient sera as in panel A. (C) Western blot of purified gonococcal TbpA and TbpB probed with patient sera diluted at 1:100. Lanes 1 and 16 are negative controls with no antibody. Lane 2 was probed with an anti-TbpA antibody. Lanes 3 through 5 are female control sera. Lanes 6 and 7 are male control sera. Lanes 8 and 9 are sera from females infected with gonorrhea for the first time. Lanes 10 and 11 contain sera from females with at least one prior gonococcal infection. Lane 12 contains sera from a male infected with gonorrhea for the first time. Lanes 13 and 14 contain sera from males with at least one prior gonococcal infection. Lane 15 was probed for TbpB by using HRP-transferrin.