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. 2004 Jan;72(1):209–218. doi: 10.1128/IAI.72.1.209-218.2004

FIG. 9.

FIG. 9.

Effect of affinity-purified IgG on opsonophagocytic killing of C. albicans serotype A. Yeast cells were incubated with PMN in the presence of affinity-purified antibody at concentrations that produced ELISA-determined titers over the range of 1/312 to 1/80,000 in the presence or absence of 50% PHS that had been absorbed with mannan-Sepharose to remove mannan antibody and MBL. Control groups comprised PMN incubated with C. albicans in the absence of pooled normal human sera (No NHS, No IgG) or in the presence of 50% concentrations of pooled normal human sera (NHS). Results are presented as the numbers of CFU remaining after a 2-h assay. Data are reported as the means ± SEM of results from three independent experiments using neutrophils from different donors. *, P < 0.05 (versus normal human sera alone); #, P < 0.05 (versus no normal human sera and no IgG).