Fig. 3.

Basic features of the cryptochrome mutants and knockdown lines used in this work are shown. (A) Schematic drawing of the OsCRY1b gene structure with the Tos17 insertion sites of cry1b mutants isolated in this work. Introns (lines) and exons (boxes) are represented. Filled boxes indicate the coding regions, while open boxes correspond to 5′- and 3′-untranslated regions. (B) Western blotting analysis of OsCRY1a, OsCRY1b and OsCRY2 in cry1b mutants and cryptochrome-deficient transgenic lines. Proteins were extracted from 3-day-old etiolated seedlings of WT (Nipponbare), cry1a-R (#16 and #18), cry1b-1, cry1b-2, cry1b-3, cry1a-R cry1b-1 (#3 and #4) and cry2-R (#1 and #5) lines. A 50 µg aliquot of protein was used for each sample analyzed. Coomassie brilliant blue-stained bands served as a loading control. The anti-CRY1b antiserum used in this study detected two bands on the blots. The upper band (marked with an arrow) corresponds to CRY1b. The lower band (marked with a star) was confirmed to be a cross-reactant between the antiserum and a protein other than CRY1b.