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. 2012 May 11;40(16):e124. doi: 10.1093/nar/gks325

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cleavage of the bulged DNA substrate by fowlpox resolvase. (A) Analysis of cleavage products by native gel electrophoresis. Products were characterized by co-migration with synthetic markers identical to each expected product. (B) Analysis of products of cleavage of the bulged DNA substrate analyzed by denaturing gel electrophoresis. The inferred sites of cleavage are shown at the bottom. ‘Mix’ indicates a mixture of the synthetic 15 nt expected cleavage product and an authentic reaction mixture, indicating co-migration with the indicated product. (C) Resolvase catalytic site mutants obstruct resolvase cleavage as measured in the FP assay. The bulged DNA substrate was mixed with D7A, D135A or wild-type resolvase, then cleavage tracked for the indicated times.