Figure 3.

(A) Left panel: Overlaps between interprobe distance PDFs derived from in-cell FRET measurements (blue line) and ‘in-cell’ PDFs simulated using FRET NPS tools for A- (red line) and B-form (green line) of DNA. Right panel: Overlaps between PDFs derived from in vitro FRET measurements (blue line) and ‘in vitro’ PDFs simulated for B-form of DNA. (B) and (C) AVs of ATTO680 (olive mesh) simulated for A-form and B-form DNA, respectively, and FRET derived and constrained positions of ATTO740 (blue spheres). Both simulations accounted for altered fluorophores properties in cellular environment. For details on the simulation approach, see ‘Materials and Methods’ section.