Table 2.

Probabilistic interpretation of the in-cell FRET data

bp	A-DNA	B-DNA
8	0.87	0.60
10	0.92	0.81
12	0.80	0.67
14	0.51	0.39
16	0.65	0.43

Quantification of a distance between the ‘in-cell’ PDFs simulated using FRET NPS tools (22) for A- and B-form of DNA and ‘reference’ interprobe distance PDFs derived from in-cell FRET measurements using the Kolmogorov–Smirnov (KS) statistic. Identical distributions are marked by 0 value in the KS statistics. Note that B-DNA model has lower KS values compared to A-DNA model for all the DNA constructs indicating the B-form of DNA to be a more likely conformation in intact bacterial cells compared to A-DNA.