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. 2012 Apr 26;40(16):e120. doi: 10.1093/nar/gks341

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Assessment of DeltaPhage in pIX display phagemid rescue. (A) Two pIX display phagemids (encoding either an anti-phOx or anti-NIP scFv) were propagated in 50 ml cultures of E. coli XL1-Blue and TOP10F′ followed by rescue by either M13K07 or DeltaPhage helper phages. Phages were precipitated and concentrated by PEG, followed by infectious titration and the results given as cfu^ampR/ml. (B) Phagemid to helper phage ratios were determined by cfu^ampR/cfu^kanR from parallel infectious titration of the same samples. The results are given as mean ± SD of three independent colonies of each sample assessed in parallel. (C) Two pIII display phagemids (encoding either the anti-phOx or anti-NIP scFv) were propagated in E. coli XL1-Blue and rescued with either M13K07, or Hyperphage. Phagemid to helper phage ratios were determined as above. The results are given as mean ± SD of three to four independent colonies of each sample assessed in parallel.