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. 2012 May 31;40(16):8021–8032. doi: 10.1093/nar/gks392

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — GlmS:GFP upregulation using C. perfringens and C. difficile Hfq variants by GlmZ and GlmY. (A) Sequence homology between E. coli, C. difficile and C. perfringens Hfq variants. Nucleic acid-binding Sm1/Sm2 domains and important amino acid residues in proximal and distal RNA-binding surfaces required for regulation are indicated. (B) Cross-complementation for Hfq^Ec using Hfq^Cp and Hfq^Cd homologues for GlmS upregulation. The ability to activate GlmS expression by GlmZ and GlmY was measured when Hfq^Cd and Hfq^Cp was supplemented in E.coli Δhfq cells.