Figure 6.

Detection of RNase MRP RNAs in wheat germ RNase P preparations. The following PCR templates were used: (A) RNAs isolated from AtPop1p-specific wheat germ immunoprecipitate. Sample 1: cDNA prepared from DNase I-treated RNA; 2: DNase I-treated RNA; 3: mock-treated RNA. Nested PCR with MRP-specific primers was performed with templates 1–3, with 10 ng genomic DNA from wheat (C1) or without template (C2). (B) RNAs prepared from purified wheat germ RNase P (Blue Trisacryl pool) were treated as described in A. M: DNA marker (Gene Ruler low range, Fermentas); relevant sizes in bp are indicated to the right. The 170 bp PCR product is specific for wheat RNase MRP RNA. The ethidium bromide stained gel is shown in inverted colour to enhance contrast.