FIG. 2.

Solid-phase ligand-binding assay showing specific Lys-plasminogen-binding (A) and Glu-plasminogen-binding (B) activities with different His-tagged recombinant SEN proteins. The assay was performed in 96-well microtiter plates. The results were analyzed by the one-site- and two-site-binding nonlinear-curve models with GraphPad Prism version 3.03 software. The best-fit curve shown is the one-site-binding curve, as validated by the F test. The extracted affinity binding values (K_d and B_max) for each curve are in Table 2. Each data point represents an average value of three experiments, each done with duplicate samples. SEN-Wt, wild-type SEN.