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. 2004 Jan;72(1):94–105. doi: 10.1128/IAI.72.1.94-105.2004

FIG. 7.

FIG. 7.

Streptococcal penetration of ECM protein-coated Trans-well membranes (3-μm pore size). GAS strains D471-M6 (WT), D471-M6-SEN-K434-435L (MUT), and D471-M6-aad (WTR) were added to the upper wells either alone or after preincubation with Lys-plasminogen (Plg). Streptococci preincubated with Lys-plasminogen were treated separately with tPA and Ska or in combination with Apl. The presence in the lower wells of streptococci that penetrated the membrane was determined by counting CFU on blood agar plates. Mean values obtained with three or four individual Trans-wells ± the standard error of the mean are shown. The inset showing the statistically significant difference between the CFU counts of two strains highlights the comparison of the effects of different treatments on the ability of GAS strains to penetrate the membrane. N.S., no statistically significant difference (P > 0.05).