TABLE 1.
Primers used in this study
| Primer | No. | Sequencea |
|---|---|---|
| sen-F-NdeI | 1 | 5′ CTACGGCCATATGTCAATAATAACTGATGTGTATGCTCG 3′ |
| sen-R-BamHI | 2 | 5′ CGCGGATCCTTTTTTTAAGTTATAGAATGATTTGATACC 3′ |
| sen-ΔK435 | 3 | 5′ CGCGGATCCTACTATTTTAAGTTATAGAATGATTTGATACC 3′ |
| sen-ΔK434-435 | 4 | 5′ CGCGGATCCTACTATAAGTTATAGAATGATTTGATACC 3′ |
| sen-K435L | 5 | 5′ CGCGGATCCTACTATAATTTTAAGTTATAGAATGATTTGATACC 3′ |
| sen-K434-435L | 6 | 5′ CGCGGATCCTACTATAATAATAAGTTATAGAATGATTTGATACCTTTG 3′ |
| sen-K428-434-435L | 7 | 5′ CGCGGATCCTATAATAATAAGTTATAGAATGATAAGAVACCTTTGTA 3′ |
| sen-DST-F | 8 | 5′ CTGCAGATTAGTCTAGUGGACTCATTTATCC 3′ |
| sen-DST-R | 9 | 5′ CATATGTGTTGAGCAACTAGCTGCTTTGACG 3′ |
| sen-F-SalI | 10 | 5′ ACGCGTCGACATG TCAATAATAACTGATGTGTATGCTCG 3′ |
| spc-1-F | 11 | 5′ GGTACTTACATGTTTGGATCAGG 3′ |
| spc-2-R | 12 | 5′ CCATTCAATATTCTCTCCAAG 3′ |
| sen-C-F | 13 | 5′ GGTGAAGTTGCUCAATACAAAGG 3′ |
| sen-DST-N-R | 14 | 5′ CACATTCTACGAATGACC 3′ |
| sen-DST-C-R | 15 | 5′ CGACAGAGCAGTTGATATCTTGG 3′ |
a
Restriction sites introduced into the primer sequences are underlined.