TABLE 2.
Affinity constants of different His-tagged SEN proteins for Lys- and Glu-plasminogena
| His-tagged sen protein | Lys-plasminogen
|
Glu-plasminogen
|
||
|---|---|---|---|---|
| Kd (nM) | Bmax (102 fmol) | Kd (nM) | Bmax (102 fmol) | |
| SEN-Wtb | 127 | 10.70 | 267 | 4.79 |
| SEN-ΔK435 | 260 | 11.0 | 600 | 2.45 |
| SEN-ΔK434-435 | 327 | 5.9 | 995 | 0.7 |
| SEN-K435L | 1,119 | 1.3 | 1,029 | 0.7 |
| SEN-K434-435L | ND | 0.6 | ND | 0.13 |
| SEN-K428-434-435L | ND | 0.5 | ND | 0.08 |
a
Kd, equilibrium dissociation constant; Bmax, maximal amount of radioligand bound per well containing the specific SEN recombinant protein; ND, undeterminable.
b
SEN-Wt, wild-type SEN.