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. 2012 May 30;40(16):7676–7689. doi: 10.1093/nar/gks509

Figure 2.

Figure 2.

IKKα interacts with HP1γ and histone H3.3. (A) IKKα kinase assay with recombinant HP1γ. Western blot has been performed with anti-IKKα, anti-HP1γS93p and anti-HP1γ. (B) IKKα kinase assay with recombinant IKKα and histone H3.3 in presence or absence of recombinant HP1γ (top) or HP1α (bottom). Western blot has been performed with anti-IKKα, anti-H3S10p, anti-H3.3S31p, anti-HP1γ and anti-HP1α antibodies. (C) eCoIP experiments with nuclear extract from RAW264.7 cells treated with LPS for 1 h. Antibodies used for immunoprecipitation are named at the top of the figure and antibodies used for western blot named on the left. Input was 5% of material used for CoIP. (D) In vitro CoIP experiment performed with recombinant HP1γ–GST or IKKα–GST incubated with or without ATP and immunoprecipitated with anti-HP1γ or anti-IKKα antibodies. Western blot performed with anti-HP1γ or anti-IKKα antibodies. (E) In vitro CoIP experiment performed with recombinant HP1α-HIS incubated with recombinant HP1γ–GST or IKKα–GST and immunoprecipitated with anti-HP1α or anti-IKKα antibodies. Western blot performed with anti-HP1α antibody.