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. 2012 May 30;40(16):7676–7689. doi: 10.1093/nar/gks509

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Transcription elongation-dependent accumulation of IKKα, HP1γ and H3.3S31 phosphorylation at the TNF locus. (A) Quantification of total IKKα, HP1γ, H3.3S31p, H3.3 and β-actin protein levels by western blot with total cell extract, after RAW264.7 cells incubation with LPS+DRB. RAW264.7 cells treated with LPS in presence or absence of DRB for 30 min LPS (30), 30 min LPS+DRB (30+DRB), 60 min LPS (60) or 30 min LPS following by 30 min with DRB (60+DRB). ChIP performed with (B) anti-IKKα and (C) anti-HP1γ. Horizontal axis indicates primers used for the real-time PCR. Data are normalized versus input and then versus an average of control regions. Data are representative of at least three independent experiments. Error bars represent standard deviation (SD) from three independent qPCR replicates.