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. 2012 May 30;40(16):7676–7689. doi: 10.1093/nar/gks509

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — HP1γ knockdown reduces chromatin-associated IKKα. HP1γ knockdown in RAW264.7 cells treated with LPS for 1 h. (A) ChIP performed with anti-RNAPII S2p and anti-HP1γ normalized versus input only. The unbound fraction of a first anti-RNAPII S2p ChIP was used as a template for a second ChIP, (B) with anti-RNAPII S2p and anti-HP1γ normalized versus the unbound fraction only, (C) with anti-HP1γ and (D) anti-IKKα. (A–D) Horizontal axis indicates primers used for the real-time PCR. (C,D) Data are normalized versus the unbound fraction (Unb) and then versus an average of control regions. Data are representative of at least five independent experiments. Error bars represent ± standard deviation (SD) from three independent qPCR replicates.