Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 30;40(16):7676–7689. doi: 10.1093/nar/gks509

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 8. — CUEDC2 accumulates at the 3′-end of TNF gene. (A) eCoIP experiment with nuclear and cytoplasmic extracts from RAW264.7 cells treated with LPS for 1 h and precipitated with anti-IKKα antibody followed by western blot with anti-HPRT, anti-PP1 and anti-CUEDC2 antibodies. (B) ChIP with RAW264.7 cells treated with LPS performed with anti-CUEDC2 antibody. Horizontal axis indicates primers used for the real-time PCR. Data are normalized versus input and then versus an average of control regions. Data are representatives of at least three independent experiments. Error bars represent ± standard deviation (SD) from three independent qPCR replicates. (C) Suggested model of IKKα cycling activity during transcription elongation. (Step 1) Both IKKα and HP1γ bind to phosphorylated RNAPII CTD. IKKα phosphorylates HP1γS93. (Step 2) The complex IKKα/HP1γS93p is transferred to chromatin. (Step 3) The presence of HP1γ favours H3.3S31 phosphorylation by IKKα. (Step 4) After the passage of the RNA polymerase, H3.3S31 is phosphorylated, unphosphorylated HP1γS93 is high and IKKα low. (transcription termination) CUEDC2 co-localizes with the IKKα/HP1γS93p complex on chromatin, whereas H3.3S31 is not phosphorylated.