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. 2012 Jun 18;40(16):8072–8084. doi: 10.1093/nar/gks510

Figure 2.

Figure 2.

NMR spectra of DrsA34 and enzymatic probing of DsrA34 with RNase V1 in the presence and absence of HfqEc, respectively. (A) NMR spectra of free DrsA34 and in complex with HfqEc65. (B) In vitro RNase V1 cleavage of DsrA34 was performed in the presence (lanes 2 and 3) and in the absence (lanes 4 and 5) of HfqEc. 7.5 × 10−3 (lanes 2 and 4) and 1.5 × 10−2 (lanes 3 and 5) units of RNase V1 were added. Lanes 1 and 6, sequence ladder obtained by alkaline hydrolysis of [32P]-5′ end-labelled DsrA34. The numbers denote nucleotide positions in DsrA34. The arrows denote RNase V1 cleavage sites on DsrA34 in the absence (C) and presence (D) of HfqEc, respectively, derived from enzymatic probing.