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. 2012 Jun 7;40(16):8129–8143. doi: 10.1093/nar/gks516

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — The effect of Ocr and variants on DNA cleavage by EcoKI. The end lanes show 1 kbp DNA size markers increasing from 2 kb to 7 kb. The pBRsk1 lanes show the uncut plasmid predominantly in the supercoiled form with a small amount of open circular form running more slowly. The EcoKI lanes show linearization of the DNA by EcoKI. The other lanes show the effect of Ocr and its variants on EcoKI activity. The enzyme was mixed with the Ocr proteins at a ratio of 1 enzyme per 10 Ocr prior to addition to the DNA sample.