Table 1.
Mutant name | Changes to wild-type amino acid sequence |
---|---|
Mut1 | D12N, E16Q, E20Q, D25N, D26N |
Mut2 | D25C, D26N, D29N, D31N, D32N, D35N |
Mut3 | E59Q, D62N, E64Q, E66Q, D67N, D73C |
Mut4 | D92N, E95Q, D96N, E98Q, D99N |
Mut12 | E59Q, D62N, E64Q, E66Q, D67N |
Mut7 | Mut2 + Mut4 |
Mut10 | Mut2 + Mut4 + Mut12 |
Mut11 | Mut2 + Mut12 |
Mut13 | Mut4 + Mut12 |
Mut16 | Mut1 + Mut12 |
Ocr99 | Deletion of C-terminal amino acids 99–116 |
Ocr109 | Deletion of C-terminal amino acids 109–116 |
Ocr/POcr | D76N, E87Q, D92N, E95Q, D96N, E98Q, D99N, E103Q, E106Q, E107Q, E109Q, E110Q, E112Q, E113Q, D114N, E115Q, E116Q |
POcr | All 34 Asp and Glu changed to Asn and Gln, respectively. |
The Ocr99 and Ocr109 variants have been described previously (14).