Table 3.

ITC analyses for the interaction of the M.EcoKI MTase with Ocr or its variants

Ocr variant	Stoichiometry Ocr dimers per M.EcoKI	Enthalpy (kcal mol−1)	Dissociation constant (Kd) (nM)
Wild typea	0.87 ± 0.01	−20.5 ± 0.3	Too tight
Mut1	0.98 ± 0.01	−13.8 ± 0.3	Too tight
Mut2	1.04 ± 0.01	−14.5 ± 0.2	Too tight
Mut3	0.93 ± 0.02	−14.7 ± 0.5	79 ± 21
Mut4	0.83 ± 0.01	−19.4 ± 0.2	Too tight
Mut7	0.99 ± 0.01	−12.5 ± 0.3	Too tight
Mut10	∼0	∼0	∼no binding
Mut11	∼0	∼0	∼no binding
Mut12	0.90 ± 0.01	−15.4 ± 0.4	20 ± 9
Mut13	1.18 ± 0.03	−11.0 ± 0.4	41 ± 16
Mut16	0.98 ± 0.03	−8.2 ± 0.4	71 ± 30
Ocr/POcr	0.89 ± 0.01	−15.3 ± 0.2	Too tight
Ocr99a	0.71 ± 0.01	−22.4 ± 0.7	Too tight
Ocr109a	0.74 ± 0.01	−25.0 ± 0.6	Too tight

^aData from (17).