Table 4.
Summary of results with Y indicating yes, N indicating no and X nM indicating the dissociation constant for binding
| Ocr variant expressed | Active versus whole RM system in vivo | Active versus M.EcoKI alone in vivo | Protection against restriction of chromosome in presence of 2AP | Normal growth in liquid culture without IPTG | Normal growth in liquid culture +IPTG | Normal cell morphology | Normal growth on solid media without IPTG | Binding to M.EcoKI in vitro | Inhibition of nuclease in vitro | Summary |
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type | Y | Y | Y | Y | Y | Y | Y | Y | Y | Normal Ocr |
| Mut1 | Y | Y | Y | Y | Y | Y | Y | Y | ||
| Mut2 | Y | Y | Y | Y | Y | Y | Y | Y | ||
| Ocr99 | Y | Y | Y | Y | Y | Y | Y | Y | ||
| Mut4 | Y | Y | Y | Y | Y | Y | Y | Y | ||
| Ocr109 | Y | N | Y | Y | Y | Y | Y | Y | ∼Normal but some odd effects | |
| Mut7 | Y | Y | N | Y | Y | Y | Y | Y | ||
| Ocr/pOcr | Y | N | N | ∼Y | N | N | N | Y | N | Weakening binding, some loss of activity. Cells sick due to lack of RA. |
| Mut3 | Y | N | N | Y | ∼N | N | ∼Y | 79 nM | N | |
| Mut12 | Y | ∼N | N | N | N | N | N | 20 nM | N | |
| Mut16 | Y | N | N | N | N | N | N | 71 nM | N | |
| Mut13 | N | N | N | Y | Y | Y | 41 nM | N | Total loss of activity and cells are well. | |
| Mut10 | N | N | N | Y | Y | Y | N | N | ||
| Mut11 | N | N | N | Y | Y | Y | N | N | ||