Figure 4.

TALENs induce high rates of heritable mutations. Fish that had been previously injected with either homodimeric or heterodimeric TALENs were screened for founders carrying heritable mutations. The targeted gene and the TALEN pairs injected are indicated at the top of each panel. Individual embryos from each potential founder were lysed for PCR analysis. Indel mutations were identified by the presence of the PCR size variants. Except for hey2 and gria3a, the sequences of the mutant alleles have been determined by sequencing (Figure 5). TALEN 1295/1260, 1297/1257 and the SQT constructs encode homodimeric TALEN pairs, whereas the WH constructs encode ELD/KKR heterodimeric TALENs. ^aThe number of embryos that possessed indel mutations are shown outside of the parentheses, and the sizes of the indels are shown inside the parentheses. Some of the mutation sequences are shown in Figure 5.