Figure 5.

miR-19b transfection increases inflammation in primary human synovial fibroblasts. RA primary FLS were transfected with 10 nM of indicated RNA duplex (siEGFPN is used as control for siRNF11, both being Dicer substrates; see ‘Materials and Methods’ section), and incubated for 15 h. Supernatants were collected and assayed for IL-6 (A) and IL-8 (B) levels. Cytokine levels were normalized to that of siEGFPN condition to remove variations between independent experiments. The data are averaged from two independent experiments in biological triplicate, with Mann–Whitney U tests compared to siEGFPN condition (*P < 0.05 and **P < 0.01). (A–B) SEM is shown.