Figure 1.
Knockdown of Sp2 impairs proliferation of HEK293 cells. (A) Experimental design of growth rate determination. A total of 0.5 × 105 HEK293 cells were plated at Day 0, 3 and 6. Transfection of siRNA was performed 16 h post-plating on Day 1, 4 and 7. (B) Knockdown efficiency of Sp2. Western blotting was performed at Day 0, 3, 6 and 9. To control for loading, the blots were re-probed for tubulin. (C) Growth rates of HEK293 cells transfected with the indicated siRNAs. Cumulative cell numbers were calculated by multiplying the number of plated cells with the fold increase in cell number at the end of each 3-day interval resulting in theoretical total numbers of proliferated cells arising from cells plated on Day 0 without contact inhibition due to restricted culture vessel size. Presentation is as mean ± SD (n = 3).