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Accumulation of major translation initiation factors in selenite-induced SGs. The localization of PABP, eIF4G (top row, red and blue, respectively), eIF4E (second row, red), eIF4A1 (third row, red) and eIF3b (bottom row, red) was probed by immunostaining of selenite-treated U2OS cells. Arrows indicate SG localization, whereas arrowheads indicate the lack of enrichment in SGs. Bar = 10 µm.
