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Effect of 4EBP1 knockdown on the viability of stressed cells. U2OS cells transfected with control or 4EBP1 siRNA were treated either with arsenite (at 100 µM for 0, 2 and 4 h; left panel) or selenite (left, at 1 mM for 1 h; right, 10 µM or 20 µM selenite for 24 h; right panel). Subsequently, cell viability was assessed by measuring the amount of intracellular ATP levels. Data represent relative viability expressed as % control relative to non-treated cells.
