Figure 4.

Identification of endogenous genes inversely regulated by BCL-6 and STAT5. (A) DLD-1 and HT29 cells were transfected with either BCL-6 or STAT5-specific siRNA oligonucleotides and lysed following 48 h for RNA extraction. (Supplementary Figure S2). A heat map display of the gene expression analysis of a cell-cycle PCR array probed with RNA samples obtained from BCL-6 or STAT5-depleted DLD-1 and HT29 cells, is shown. Of the 84 genes on the array, 3 were identified to be regulated by BCL-6 and STAT5 in opposite sense (white boxes). (B) Schematic representation of the promoter regions of the three endogenous genes inversely regulated by BCL-6 and STAT5 showing the selected best putative motifs, with equivalent predicted binding scores for both factors.