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Silver stain of NTHi 2019 LOS from planktonic, biofilm, and plate cultures. LOS was prepared from broth, plate, and continuous-flow tube biofilm cultures and examined by Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining (see Materials and Methods). Reactivity with MAb 3F11 is an index of asialylated terminal lactosamine and was determined by dot blot analysis.
