TABLE 2.
Quantitation of bacteria recovered from a silicon tube flow biofilm growth assaysa
| Strain | Growth medium | Bacteria recovered (log10 no. of CFU) |
|---|---|---|
| NTHi 2019 | sBHI | 11.73 ± 2.04 |
| NTHi 2019 siaB | sBHI | 5.96 ± 1.34 |
| NTHi 2019 | CDM | 6.34 ± 1.89 |
| NTHi 2019 | CDM + NeuAc | 8.49 ± 3.46 |
a
Bacteria were cultured in supplemented BHI (sBHI) or a chemically defined minimal medium (CDM) (26) for 24 h. NeuAc (20 μg/ml) was added as indicated. H. influenzae was inoculated into sterile silicon tubing, through which a continuous flow of growth medium was passed (50). After 24 h, the tubing was excised and the numbers of CFU were obtained (see Materials and Methods).