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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Free Radic Biol Med. 2011 Dec 29;52(6):1067–1074. doi: 10.1016/j.freeradbiomed.2011.12.016

Fig. 3.

Fig. 3

15d-PGJ2 reduces Mn-induced activation of NF-κB in astrocytes. NF-κB expression was examined by the detection of the p65 subunit. Astrocytes were pretreated for 2 h with (A) 15 d-PGJ2 (10 µM) or (B) LY29004 (20 µM) before treatment with Mn (500 µM) for 6 h. Nuclear fractions were immunoblotted for the NF-κB p65 subunit. (C) Intracellular localization of NF-κB was examined by the detection of p65 with immunocytochemistry upon treatment with Mn, 15 d-PGJ2, or 15d-PGJ2 plus Mn for the indicated incubation time periods. Data are means±SEM (n=4) (A, B). Imaging sections are representative of six examined sections. ***p<0.001 compared to the controls, #p<0.05, ###p<0.001 compared to the matched groups, @@@p<0.01.