15d-PGJ2 attenuates Mn-induced inhibition of DJ-1 expression in astrocytes. (A) Top: astrocyte lysates were fractionated into nuclear, cytosolic, and mitochondrial fractions. Bottom: astrocytes were incubated with Mn (500 µM) for various times to analyze DJ-1 protein levels. (B, C) Astrocytes were pretreated for 2 h with 15 d-PGJ2 (10 µM) before Mn (500 µM) exposure for 6 h, followed by (B) Western blot or (C) RT-PCR analyses. (D) Astrocytes were also pretreated for 1 h with LY294002 (20 µM) before Mn (500 µM) exposure for 6 h. Data are means±SEM (B, n=4). ***p<0.001 compared to the controls; ###p<0.001 compared to the matched groups; @@p<0.01.