Table 1.

Summary viability of E. coli

	5 h					24 h
	+ IPTG		No IPTG		Inhibition index	+ IPTG		No IPTG		Inhibition index
	LBa	LB	LBa	LB		LBa	LB	LBa	LB
No T7 promoter	452	428	447	463	1.0  ± 0.1	114	143	188	154	1.4  ± 0.1
	424	406	356	472		120	112	170	165
E. coli C5 32/−2	176	137	278	295	1.5  ± 0.2	5	4	86	79	15  ± 2.0
	157	319	282	283		4	8	84	81
EGyr241/211	61	71	250	290	4.2  ± 0.2	1	3	62	43	12  ± 6.0
	69	58	251	281		5	8	48	35
EGyr241/223	78	71	302	309	3.8  ± 0.5	4	7	37	43	7.0  ± 0.5
	62	110	301	284		7	4	53	34
EGyr242/211	184	136	346	310	1.9  ± 0.2	10	13	65	59	5.6  ± 0.3
	193	159	307	298		11	7	51	59
EGyr242/223	149	117	281	286	2.2  ± 0.2	7	4	74	48	14  ± 2.0
	136	108	263	265		4	4	70	73

Colony counts of individual plates are listed for quantitative plate cultures from a single assay in vivo performed as described in Materials and Methods. The E. coli transformant panel included a negative control (no T7 promoter) described in Fig. 4 and text. Other transformants encoded dual EGS constructs against either E. coli C5 (E. coli C5 32/−2) or EGyr A (EGyr241/211, etc.), named according to the EGS nomenclature described in Fig. 1 and the text. Quantitative plating was performed at two times: 5 and 24 h after liquid cultures were split for parallel incubation either with induction (+ IPTG) or without induction (no IPTG) for EGS expression. Duplicate plates are listed for both LB and LBa agar plates. An inhibition index is calculated (see text) from the ratio of the colony counts of a given transformant without EGS induction versus with EGS induction, such that an inhibition index of 1 corresponds with no decrease in colony counts after EGS induction, and an inhibition index of 5 corresponds with a 5-fold decrease in colonies with EGS induction as compared to without EGS induction.