HF n-3 PUFA diet lowered activity. (A) Ambulatory, (B) rearing, and (C) running wheel activities as a function of time for mice fed differing diets. Corresponding average light and dark cycle (D) ambulatory, (E) rearing and (F) running wheel activities. Counts represent the number of laser beams broken due to movement in the cage. Data are mean ± SEM, n = 7 mice per diet. Significance during the dark cycle compared to CD is indicated by *P < 0.05, †P < 0.01, ‡P < 0.001.