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. 2012 Apr 2;5(5):984–992. doi: 10.1093/mp/sss032

Table 1.

Quantification of Xyloglucanase-Released Xyloglucan Oligosaccharides.

Genotype XXXG XXFG XLXG XXLG XLFG Gal at 3rda Total Fb Total XyG
Wild-type (Col-0) 0.30 ± 0.04c 0.12 ± 0.02 0.04 ± 0.02 0.27 ± 0.05 0.04 ± 0.01 0.42 ± 0.08 0.16 ± 0.03 0.76 ± 0.14
mur3.1 0.23 ± 0.04 0.00 ± 0.00 0.18 ± 0.04 0.01 ± 0.01 n.d. 0.02 ± 0.01 0.00 ± 0.00 0.42 ± 0.09
xlt2 0.18 ± 0.05 0.22 ± 0.04 n.d. 0.05 ± 0.01 n.d. 0.28 ± 0.06 0.22 ± 0.04 0.46 ± 0.11
XLT2_Comp 0.27 ± 0.04 0.09 ± 0.02 0.12 ± 0.02 0.31 ± 0.05 0.05 ± 0.02 0.45 ± 0.09 0.14 ± 0.04 0.84 ± 0.15
mur3.1 xlt2 0.35 ± 0.06 0.01 ± 0.01 0.00 ± 0.00 0.00 ± 0.00 n.d. 0.02 ± 0.01 0.01 ± 0.01 0.36 ± 0.06
a

Sum of oligosaccharides with galactose at the third position (XXLG, XXFG, XLFG).

b

Sum of fucosylated oligosaccharides (XXFG, XLFG).

c

XyG oligosaccharide quantification based on HPAEC–PAD using an XXXG standard. Quantities are μg mg−1 of AIR prepared from rosette leaves. n = 4; n.d. - not detected.