FIG. 5.
Inhibition of HIV-1 replication in 2LTRZFP-GFP-transduced T-lymphoid cells. SupT1 cells were transduced with either 2LTRZFP-GFP or Aart-GFP, using third-generation lentiviral expression vectors. The following stable lines were propagated: Aart-10F, ZFP-5E3, and ZFP-4F6. These cells were infected with HIV-1NL4-3 at 0 and 1 MOI. (A) Comparison of HIV-1 p24 antigen in the culture supernatants of these cells on day 13 after challenge. The level of p24 antigen in the culture supernatants of ZFP-5E3 was significantly higher than in ZFP-4F6 (*p<0.05, Mann–Whitney U test). This graph is representative of two independent experiments. (B) Comparison of the mean Ct values±SD of Alu-gag qPCR and GAPDH qPCR of extracted genomic DNA from these clones on day 13 after challenge. The PCR assays were performed in triplicate.