FIG. 7.
Inhibition of HIV-1 integration in PBMCs expressing 2LTRZFP-GFP. PBMCs were transiently transfected with pTriEx-4-2LTRZFP-GFP, pTriEx-4-Aart-GFP, and pTriEx-4-GFP. These cells were infected with HIV-1NL4-3 at 1 MOI. Raltegravir-treated cells were used as a control for inhibition of HIV integration. (A) Flow cytometric analysis of GFP expression, representing the efficiency of transfection. (B) Comparison of the mean Ct values±SD of Alu-gag qPCR and GAPDH qPCR of extracted genomic DNA from the indicated infected cells on day 4 after challenge. The PCR assays were performed in triplicate. NA, below the detection threshold. (C) Cell viability was determined with PrestoBlue cell viability reagent.