FIG. 1.

Schematic diagram of MLV-based vectors. The MLV-based gamma-retroviral vector LZRS was used to express either green fluorescent protein (GFP), the therapeutic single chain fragment (scFv) intrabody (Leader.huTat2.Cκ.HA), or the control scFv intrabody (Leader.A3H5.Cκ.Flag); the intrabody fusion proteins used the human IgG leader to direct expression to the endoplasmic reticulum, the common kappa region (Cκ) to increase stability, and either the human influenza hemagglutinin (HA) protein (YPYDVPDYA) or FLAG epitope (DYKDDDDK) to tag protein expression. The universal primers and probe (Univ, striped arrows), located in the Psi packaging region of each vector, monitor total transduction frequencies. The vector-specific forward primer was located in the Cκ sequence downstream of the intrabody gene (grey arrow) and the vector-specific reverse primers were located in either the HA or FLAG sequences (grey arrow). Color images available online at www.liebertonline.com/hum