FIG. 5.

The percentage and phenotypic characterization of CD4⁺ T cells in PBMCs following SHIV challenge. (A) PBMCs, collected from rhesus macaques (306.98 left panel; 239.96 right panel) at the designated time points post reinfusion, were stained with anti-CD3, anti-CD4, anti-CD8, and anti-CD20 antibodies to determine the percentage of CD4⁺ (red/gray triangle) and CD8⁺ (green diamond) T cells by flow cytometry. Animals were challenged with SHIV 89.6p 1 week post reinfusion. (B) PBMCs were stained with anti-CD4, anti-CD28, and anti-CD95 antibodies at serial time points post SHIV infection, and the percentage of naïve (red/gray triangle), central memory (green open triangle), and effector memory (black triangles) cells determined by flow cytometry. Color images available online at www.liebertonline.com/hum