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MAb E492/G1 recognizes a carbohydrate epitope present in Em492 antigen. (A) ELISA wells were coated with purified Em492 antigen, and fractions were treated either with proteinase K or with 50 mM NaIO4. Note the significant decrease in MAb E492/G1 reactivity upon removal of carbohydrates. (B) Control experiment with a recombinant protein antigen (NcMIC3) and respective anti-NcMIC3 antibodies. Note the decrease in reactivity of anti-NcMIC3 upon protease digestion.
