FIG. 6.

Impairment of splenocyte proliferative responses by the addition of Em492 antigen. Splenocytes of uninfected mice (black columns), primarily infected mice (grey columns), and secondarily infected mice (dotted columns) were cultured in vitro. They were left unstimulated (control), or proliferation was induced by the mitogen ConA or with crude E. multilocularis extract (Emc). Stimulation with purified Em492 antigen did not result in any proliferation at all, and splenocyte proliferation was severely inhibited by the addition of Em492 antigen to ConA (ConA+Em492) or to Emc (Emc+Em492). Data presented are the means of quadruplicate experiments plus standard deviations.